The title of this episode is a pun on how Dee Dee is Dexter's older sister A.K.A. his big sister, hence the title of this episode.
This episode marks the debut of the Robo-Dexo 2000.
The way Dee Dee grows really huge and destroys the city is similar to the way Godzilla did in the Godzilla movies.
When Mom enters Dexter's bedroom and is met by Dexter's robot duplicate, a Power Rangers-like poster can be seen on Dexter's bedroom wall.
Dexter imagines himself getting a phone call from God, congratulating him on saving the world.
This is one of the few times there has been a religious reference in Dexter's Laboratory.
In a part of the episode, there was an interview with the President of the USA. He was not seen and was only heard. It is most likely that this was Bill Clinton, because he was president when this episode was made and his voice is clearly a parody of Bill Clinton's.
This would be the first and one of three times the President of the USA is mentioned in the show. The second time being in "Critical Gas" (where he was actually seen rather than heard or mentioned and he was clearly shown to be Bill Clinton) and the third time being in "Let's Save the World You Jerk!".
When Dexter's mom enters his room, there is a reference to the five original rangers (red, yellow, pink, blue and black) of the 90's show Mighty Morphin Power Rangers. The Power Rangers franchise has grown a following since its debut and has produced collectible merchandise.
The experimental cookies are very similar to the Eat Me Cookies from Alice in Wonderland.
At the beginning of the episode, the plate of cookies was seen inside of Dexter's Laboratory, but later in the episode, it was seen in his bedroom.
At the end of the episode, Mom had turned into a giant mutant because she ate the cookies and she saw what happened. This means that she had figured out about Dexter's Laboratory and its secrets. However, she seems to have still remained oblivious to it in the later episodes.
This pilot is produced in 1995 according to the credits.
This is the second pilot of Dexter's Lab, and it aired during the What A Cartoon! Show.
This episode can be seen as an extra on a Scooby Doo video.